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Promega halotag® mammalian pull-down systems
Halotag® Mammalian Pull Down Systems, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/halotag+mammalian+pull-down+system/halotag+mammalian+pull+down+system/pmc12207472-209-11-13
Average 90 stars, based on 1 article reviews
halotag® mammalian pull-down systems - by Bioz Stars, 2026-09
90/100 stars

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Pull Down Assay:

Article Title: Potential therapeutic target secretogranin II might cooperate with hypoxia-inducible factor 1α in sunitinib-resistant renal cell carcinoma.
Article Snippet: See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C om m ons L icense | 3949FUKUMOTO et al. software, version 5.0 (SAS Institute, Inc.). p values less than 0.05 were accepted as statistically significant. .. The HaloTag pull- down assay was carried out using the HaloTag Mammalian Pull- Down System (Promega) according to the manufacturer's instructions. .. Briefly, HaloTag SCG2 Vector (HaloTag ORF Clone FHC12720) or HaloTag control vector (mock sample) were transfected into human ACHN cells by using FuGENE 4K (Promega) according to the manufacturer's instructions.

Article Title: Potential therapeutic target secretogranin II might cooperate with hypoxia‐inducible factor 1α in sunitinib‐resistant renal cell carcinoma
Article Snippet: All analyses were carried out using expert StatView software, version 5.0 (SAS Institute, Inc.). p values less than 0.05 were accepted as statistically significant. .. The HaloTag pull‐down assay was carried out using the HaloTag Mammalian Pull‐Down System (Promega) according to the manufacturer's instructions. .. Briefly, HaloTag SCG2 Vector (HaloTag ORF Clone FHC12720) or HaloTag control vector (mock sample) were transfected into human ACHN cells by using FuGENE 4K (Promega) according to the manufacturer's instructions.

Article Title: Perfluorooctane sulfonate induces autophagy-dependent lysosomal membrane permeabilization by weakened interaction between tyrosinated alpha-tubulin and spinster 1.
Article Snippet: Perfluorooctane sulfonate (PFOS) is one kind of persistent organic pollutants.. In previous study, we found that PFOS induced autophagy-dependent lysosomal membrane permeabilization (LMP) in hepatocytes, and siRNA against lysosomal permease spinster 1 (SPNS1) relieved PFOS-induced LMP.. However, whether and how SPNS1 functioned as the link between autophagy and LMP was still not defined.

Transfection:

Article Title: GATA6 is predicted to regulate DNA methylation in an in vitro model of human hepatocyte differentiation
Article Snippet: HaloTag-fused- TET1 (FHC23878, Promega Corporation), -TET2(FHC22012, Promega Corporation), or -TET3(FHC30888, Promega Corporation) was co-transfected with pCDNA3.2-GATA6 to HEK293T cells (BRC) using Fugene HD (Promega Corporation). .. After 2 days from the transfection, the cells were collected and subjected to the HaloTag ® Mammalian Pull-Down System (Promega Corporation) according to the manufacturer’s instruction. .. The obtained sample were subjected to SDS-PAGE using NuPAGE Bis-Tris Gel (Thermo Fisher Scientific), followed by transfer to a PVDF membrane.

other:

Article Title: FAM193A is a positive regulator of p53 activity
Article Snippet: HaloTag Mammalian Pull-Down System , Promega , Cat#G6504.

Article Title: Synergistic action of human RNaseH2 and the RNA helicase-nuclease DDX3X in processing R-loops.
Article Snippet: Cellular pull-down assay was initially carried out using the Halotag mammalian pull-down system (Promega) following manufacturer’s instructions with few modifications.

Article Title: Synergistic action of human RNaseH2 and the RNA helicase-nuclease DDX3X in processing R-loops
Article Snippet: Cellular pull-down assay was initially carried out using the Halotag mammalian pull-down system (Promega) following manufacturer's instructions with few modifications.



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